Coding

Part:BBa_K2557050:Design

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-11)


TetR with TEV cleavage site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 361


Design Notes

We performed codon optimization to adapt to eukaryotic cells. In order to get TetR into the nucleus, we also added SV40 NLS.


Source

We found the sequence information of BBa_K2450202 and synthesized the sequence.

References